The Spanish giants face off again, this time in the Champions League Semi-Final.
Real have the momentum having beaten their great rivals 1-0 to win the Spanish equivalent of the FA Cup, the Copa del Rey, last week.
The win also came only four days after the sides drew 1-1 at the Bernabeu in La Liga, halting a run of five consecutive wins for the Catalan side.
It also gave Jose Mourinho, who is looking to become the first man to coach three different teams to the Champions League title, his first trophy at Real.
The Spanish sides are joint top scorers in the Champions League with 24 goals apiece, but their previous two meetings have been cautious affairs with Mourinho packing his midfield and employing more aggressive tactics.
However, the nine-times winners of European football's most sought-after trophy have had a man sent off in each of their last three games against Barca.
Team News
Barcelona midfielder Andres Iniesta will miss Wednesday's Champions League semi-final first leg against Real Madrid because of a calf strain.
Skipper Carles Puyol is fit after a muscle strain and fellow defender Gabriel Milito also plays but but full-back Maxwell (groin) misses out.
Real also have defensive issues, with centre-back Ricardo Carvalho suspended.
Injured midfielder Sami Khedira is set to be replaced by Lassana Diarra for the third El Clasico in 12 days.
Line Ups
Real Madrid
Casillas
Arbeloa Ramos Albiol Marcelo
Diarra Pepe Alonso
Ozil Di Maria
Ronaldo
Barcelona
Valdes
Alves Pique Busquets Puyol
Keita Macherano
Xavi
Messi Pedro
Villa