Gareth Bale is out of Wales' Euro 2012 qualifier against England at the Millennium Stadium on Saturday after suffering a hamstring injury.
The Football Association of Wales says in a statement the injury was suffered before Bale joined the Wales squad.
The 21-year-old Tottenham Hotspur winger did not train on Wednesday.
"Due to a hamstring problem, Gareth Bale has had to withdraw from the Wales squad to face England," a Football Association of Wales statement said.
"He felt tight last week during training with his club.
"After playing 90 minutes on Saturday he felt some muscle tightness. Initially, this was expected to be muscle soreness after playing his first game.
"On joining up with the Welsh squad he was kept off his feet in training for two days to recover.
"As he still felt tight in the warm-up on Tuesday, Gareth was pulled out of the session.
"He did not train at all on Wednesday and was sent for a scan.
"The scan revealed that Gareth Bale picked up his injury last week."
Bale has only recently returned to action for Spurs after a six-week lay-off with a back injury.
His absence is a major blow to boss Gary Speed's preparations but a boost to England who see Bale as one of their main threats.
"Gareth is top class and he can really hurt you - it's a shame at times he is not English," said 42-times capped England striker and Tottenham team-mate Peter Crouch.
Speed had hoped he could unleash "match winners" Bale and Arsenal midfielder Aaron Ramsey on Fabio Capello's side.
Ramsey is fit though and is hoping to feature for Wales for the first time since suffering a double leg break playing for Arsenal in February 2010.
http://news.bbc.co.uk/sport1/hi/football/league_of_wales/9424427.stm
The Football Association of Wales says in a statement the injury was suffered before Bale joined the Wales squad.
The 21-year-old Tottenham Hotspur winger did not train on Wednesday.
"Due to a hamstring problem, Gareth Bale has had to withdraw from the Wales squad to face England," a Football Association of Wales statement said.
"He felt tight last week during training with his club.
"After playing 90 minutes on Saturday he felt some muscle tightness. Initially, this was expected to be muscle soreness after playing his first game.
"On joining up with the Welsh squad he was kept off his feet in training for two days to recover.
"As he still felt tight in the warm-up on Tuesday, Gareth was pulled out of the session.
"He did not train at all on Wednesday and was sent for a scan.
"The scan revealed that Gareth Bale picked up his injury last week."
Bale has only recently returned to action for Spurs after a six-week lay-off with a back injury.
His absence is a major blow to boss Gary Speed's preparations but a boost to England who see Bale as one of their main threats.
"Gareth is top class and he can really hurt you - it's a shame at times he is not English," said 42-times capped England striker and Tottenham team-mate Peter Crouch.
Speed had hoped he could unleash "match winners" Bale and Arsenal midfielder Aaron Ramsey on Fabio Capello's side.
Ramsey is fit though and is hoping to feature for Wales for the first time since suffering a double leg break playing for Arsenal in February 2010.
http://news.bbc.co.uk/sport1/hi/football/league_of_wales/9424427.stm
Last edited: